It seems that even with NOD32, scanning something that I know to be infected, doesn't always come back with a hit. Sometimes I'll try extracting the .exe with 7zip and seeing if the files seen suspicious. Also when running a suspected infected file, I'll make sure to block access with Comodo.
What are some good methods you use to determine if a file should not be clicked on?
What are some good methods you use to determine if a file should not be clicked on?