XFX 9400GT will it work in a pci-express 1x slot?

Technically yes. In practice, it will drag down your FPS more than just having your main video card do the PhysX work. If your main video card is a 9600GT as it says in your sig, then you shouldn't be bothering with a PhysX card.

When you upgrade from the 9600GT, keep it around, and use that for PhysX, because that is the minimum card that will run PhysX fast enough to be useful.

PCI-E 1x slot will probably be an unacceptable bottleneck for PhysX, but I'm not sure I've seen anyone actually test that yet.
 
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